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ProCHO™ Protein-free CHO Media were developed specifically to facilitate the production and downstream processing of recombinant proteins expressed in CHO cells. These proteinfree formulations support high-density cultures without the need for animal derived components. Very low levels of recombinant insulin facilitate both downstream purification and regulatory compliance. The following media systems are available:

ProCHO™-4 - For concurrent transition of adherent CHO cells to serum-free and suspension culture. Supports faster doubling times. Cat. No. 12-029Q contains phenol red and 0.1% Pluronic® F-68. Does not contain L-glutamine, hypoxanthine, or thymidine. Cat. No. 04-919Q is similar to 12-029Q except that it does not contain phenol red.

ProCHO™-5 - For CHO cells already growing in suspension. Supports increased protein production. Contains 0.1% Pluronic® F-68. Does not contain L-glutamine, phenol red, hypoxanthine, or thymidine.

ProCHO™-AT - For adherent culture of CHO cells. Contains L-glutamine. Does not contain hypoxanthine or thymidine.

ProMEDIA Select™ CHO Media Optimization Kit contains 16 distinct formulas that contain no materials of animal origin. It can be used to develop the most optimized formulas for protein expression in CHO cells. Media formulations are scalable to production volumes.

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